Advances in Enzymology and Related Areas of Molecular by Alton Meister

By Alton Meister

A continual authoritative sequence reviewing learn into enzymology and similar parts of molecular biology. provides six papers via prime gurus.


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Additional info for Advances in Enzymology and Related Areas of Molecular Biology, Volume 55

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No ‘“C-labeled phaseollin-like products were recovered. Pretreatment of cell cultures with 4 pg ml-’ phaseollin did not protect them from the toxic effects of higher concentrations of the phytoalexin (222), as might have occurred had the host turnover system been inducible. Metabolism of exogenous phaseollin has also been observed in cell suspension cultures of Phuseolus uureus, which are not themselves able to synthesize this phytoalexin (222). In contrast, n o evidence was obtained for the metabolism of exogenously added [ ‘‘C]pisatin (XI)by leaf or endocarp tissue of Pisum sufivum (67).

This could then further condense with another molecule of acetyl CoA to yield enzyme-bound HMG (325). However, although [ ''C]malonyl CoA was incorporated into isoprenoids by enzyme preparations from yeast, pigeon, and rat liver, and Hevea brasiliensis latex, the incorporation was directly related to the presence of malonyl-CoA decarboxylase activity in the preparations (326). Moreover, the labeling pattern of ergosterol formed by the yeast preparation was also indicative of decarboxylation prior to incorporation (326).

This transferase is clearly involved in the final stages of biosynthesis of formononetin (XVI) and biochanin A (XVII) in C. uriefinum. In cell suspension cultures of GIycine may, two distinct classes of O-methyl transferase enzymes have been characterized (207). The enzymes were distinguished by differences in stability upon storage, gel chromatographic properties, changes in specific activity during the growth cycle, and substrate specificity. One enzyme, the Sadenosyl methionine :caffeic acid O-methyl transferase (CMT), catalyzed the formation of ferulic (XXVIII) and sinapic (XXIV) acids from caffeic (XXII) and 5-hydroxy ferulic acids, respectively (208).

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